rabbit monoclonal antibodies against cd44  (Boster Bio)

Journal: Nature Communications

Article Title: Longitudinal plasma proteome profiling reveals the diversity of biomarkers for diagnosis and cetuximab therapy response of colorectal cancer

doi: 10.1038/s41467-024-44911-1

Figure Lengend Snippet: A Association of proteomic subtypes with therapy response (two-sided Fisher’s exact test). B Correlation of the pathways of the G-III subtype (two-sided Pearson’s correlation test). C , E Boxplots for immune score among G-I ( N = 24), G-II ( N = 34), and G-III ( N = 31) ( P = 0.042) ( C ), and CD8+Tem score between S and NS groups in plasma samples (N (S) = 16, N (NS) = 15; P = 0.035) and tissue samples (N (S) = 12, N (NS) = 19; P = 0.005) (two-sided Student’s t test). D Differential cell types between G-III and other subtypes (two-sided Student’s t test). F Qualification of CD44 ( P = 1.0E-5) and GZMK ( P = 8.0E-6) stained by IHC in representative examples (two-sided Student’s t test). Data are shown as mean ± SD ( n = 3 independent experiments). G Association between CD8+Tem score (Z score) with tumor size (cm), and S/NS group. H Correlation of CD8+Tem score and tumor size (two-sided Pearson’s correlation test). Data are presented as Pearson r with 95%CI. I Diagram showing the potential mechanism of cetuximab sensitivity. The little heatmap depicted the log-transformed fold-change of S versus NS groups. J Correlation of CD8+Tem score and RPTOR/IMPDH2 (two-sided Pearson’s correlation test). K Kaplan–Meier curves of Overall survival (OS) in the CPTAC cohort (two-sided log rank test). L Differential expression of RPTOR ( P = 8.9E-5, 2.9E-5) and IMPDH2 ( P = 4.2E-8, 3.4E-6) in discovery cohort (N (S) = 31, N (NS) = 45) and validation cohort (N (S) = 16, N (NS) = 15) (two-sided Wilcoxon rank-sum test). PRM, parallel reaction monitoring. Boxplots show median (central line), upper and lower quartiles (box limits), 1.5×interquartile range (whiskers) ( C , E , and L ). Source data are provided as a file.

Article Snippet: A standard IHC protocol was followed to stain the tumor tissue samples of the CD8+ Tem markers by using the rabbit monoclonal antibody against CD44 (1:200, Signalway Antibody, catalog No: 48911-1), and the rabbit polyclonal antibody against GZMK (1:300, Signalway Antibody, catalog No: 40985-1).

Techniques: Clinical Proteomics, Staining, Transformation Assay, Quantitative Proteomics, Biomarker Discovery, Targeted Proteomics

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: CMG2 High cells exhibit properties of GCSLCs. a , b qRT-RCR and western blotting showing higher CMG2 level of sphere-forming cells (SC) compared to monolayer cells (MC) in SGC7901 and XN0422 cell lines; ** P < 0.01. c qRT-PCR showing upregulated stemness-related transcription factor genes Nanog, Oct4, and Sox2 expressed by CMG2 High cells compared to CMG2 Low cells; * P < 0.05. ** P < 0.01. d Higher colony forming ability of CMG2 High cells than CMG2 Low cells; ** P < 0.01. e Limiting dilution showing increased sphere formation by CMG2 High cells compared to CMG2 Low cells; * P < 0.05. f Supplemented with 10% FBS for 72 h, CMG2 High cells expressing increased differentiation markers CK18 and H-KATPase after culture with medium. g Formation of subcutaneous xenograft tumors by CMG2 High cells than CMG2 Low cells. h Increased weight of xenograft tumors formed by CMG2 High cells compared to CMG2 Low cells. i The xenograft tumors derived from CMG2 High GC cells showing higher expression of CMG2, CD44, and Ki67 compared to that derived from CMG2 Low cells. Scale bar = 50 μm

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Derivative Assay

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: CMG2 High cells are detected in CD44 High population in GC cell lines and in GC tissues. a Representative flow histogram of the percentage of CD44 High cells in SGC7901 and XN0422 cell lines. b Limiting dilution showing increased capacity of sphere formation by CD44 High cells compared to CD44 Low cells; * P < 0.05. c Representative immunofluorescence images (left panel) and quantitative statistic results (right panel) showing more CMG2-positive cells detected in CD44 High subpopulation than CD44 Low subpopulation; Scale bar = 50 μm; ** P < 0.01. d The co-localization of CMG2 and CD44 in the frozen sections of fresh GC tissues; Scale bar = 100 μm

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Immunofluorescence

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: Silencing CMG2 reduces GCSLC population. a Decreased expression of stemness marker CD44 and stemness-related factor SOX2 in GC cells with CMG2 knockdown (shCMG2). b Representative flow cytometric histograms showing decreased percentage of CD44 High cells in shCMG2 GC cells. c Markedly reduced sphere formation by CMG2 knockdown GC cells; * P < 0.05. d Decreased colony formation by shCMG2 cells. e Representative images showing decreased tumor formation in nude mice by CMG2 knockdown GC cells. f Reduced weight of xenograft tumor formed by shCMG2 GC cells; ** P < 0.01

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Expressing, Marker, Knockdown

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: Silencing CMG2 results in reduced invasive and metastatic properties of GC cells epithelial–mesenchymal transition (EMT). a Representative images of transwell invasion test showing decreased invasion capability of GC cells with knockdown of CMG2. b Quantification of the transwell invasion assay results; Scale bar = 100 μm; ** P < 0.01. c Representative images of intraperitoneal metastasis tests showing reduced number of metastatic foci formed by shCMG2 cells. d Quantification of the intraperitoneal metastasis results; ** P < 0.01. e Western blot showing upregulation of E-cadherin and downregulation of CD44 and vimentin in GC cells with knockdown of CMG2

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Knockdown, Transwell Invasion Assay, Western Blot

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: CMG2 interacts with LRP6 to activate β-catenin pathway in GC cells. a Representative immunofluorescence images showing co-localization of CMG2 and LRP6 in GC cells; Scale bar = 50 μm. b Co-IP confirmation of physical interaction between CMG2 and LRP6. c Western blotting showing reduced level of nuclear β-catenin in shCMG2 GC cells. d TOP/FOP flash showing decreased transcriptional activity of TCF4 in GC cells with silenced CMG2 (left panel) but increased transcriptional activity of TCF4 in GC cells with overexpressed CMG2 (left panel); * P < 0.05. e Western blot showing attenuated nuclear β-catenin level siLRP6 GC cells. f TOP/FOP flash showing decreased transcriptional activity of TCF4 in siLRP6 GC cells; ** P < 0.01. g Treatment with XAV-939, a specific inhibitor of β-catenin, abolishing CMG2 overexpression-induced TCF4 transcriptional activity; ** P < 0.01. h Treatment with XAV-939 decreasing nuclear accumulation of β-catenin, downregulating CD44 and vimentin, and upregulating E-cadherin in overCMG2 GC cells

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Immunofluorescence, Co-Immunoprecipitation Assay, Western Blot, Activity Assay, Over Expression