rabbit monoclonal antibodies against cd44  (Boster Bio)

Journal: Nature Communications

Article Title: Longitudinal plasma proteome profiling reveals the diversity of biomarkers for diagnosis and cetuximab therapy response of colorectal cancer

doi: 10.1038/s41467-024-44911-1

Figure Lengend Snippet: A Association of proteomic subtypes with therapy response (two-sided Fisher’s exact test). B Correlation of the pathways of the G-III subtype (two-sided Pearson’s correlation test). C , E Boxplots for immune score among G-I ( N = 24), G-II ( N = 34), and G-III ( N = 31) ( P = 0.042) ( C ), and CD8+Tem score between S and NS groups in plasma samples (N (S) = 16, N (NS) = 15; P = 0.035) and tissue samples (N (S) = 12, N (NS) = 19; P = 0.005) (two-sided Student’s t test). D Differential cell types between G-III and other subtypes (two-sided Student’s t test). F Qualification of CD44 ( P = 1.0E-5) and GZMK ( P = 8.0E-6) stained by IHC in representative examples (two-sided Student’s t test). Data are shown as mean ± SD ( n = 3 independent experiments). G Association between CD8+Tem score (Z score) with tumor size (cm), and S/NS group. H Correlation of CD8+Tem score and tumor size (two-sided Pearson’s correlation test). Data are presented as Pearson r with 95%CI. I Diagram showing the potential mechanism of cetuximab sensitivity. The little heatmap depicted the log-transformed fold-change of S versus NS groups. J Correlation of CD8+Tem score and RPTOR/IMPDH2 (two-sided Pearson’s correlation test). K Kaplan–Meier curves of Overall survival (OS) in the CPTAC cohort (two-sided log rank test). L Differential expression of RPTOR ( P = 8.9E-5, 2.9E-5) and IMPDH2 ( P = 4.2E-8, 3.4E-6) in discovery cohort (N (S) = 31, N (NS) = 45) and validation cohort (N (S) = 16, N (NS) = 15) (two-sided Wilcoxon rank-sum test). PRM, parallel reaction monitoring. Boxplots show median (central line), upper and lower quartiles (box limits), 1.5×interquartile range (whiskers) ( C , E , and L ). Source data are provided as a file.

Article Snippet: A standard IHC protocol was followed to stain the tumor tissue samples of the CD8+ Tem markers by using the rabbit monoclonal antibody against CD44 (1:200, Signalway Antibody, catalog No: 48911-1), and the rabbit polyclonal antibody against GZMK (1:300, Signalway Antibody, catalog No: 40985-1).

Techniques: Clinical Proteomics, Staining, Transformation Assay, Quantitative Proteomics, Biomarker Discovery, Targeted Proteomics

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: CMG2 High cells exhibit properties of GCSLCs. a , b qRT-RCR and western blotting showing higher CMG2 level of sphere-forming cells (SC) compared to monolayer cells (MC) in SGC7901 and XN0422 cell lines; ** P < 0.01. c qRT-PCR showing upregulated stemness-related transcription factor genes Nanog, Oct4, and Sox2 expressed by CMG2 High cells compared to CMG2 Low cells; * P < 0.05. ** P < 0.01. d Higher colony forming ability of CMG2 High cells than CMG2 Low cells; ** P < 0.01. e Limiting dilution showing increased sphere formation by CMG2 High cells compared to CMG2 Low cells; * P < 0.05. f Supplemented with 10% FBS for 72 h, CMG2 High cells expressing increased differentiation markers CK18 and H-KATPase after culture with medium. g Formation of subcutaneous xenograft tumors by CMG2 High cells than CMG2 Low cells. h Increased weight of xenograft tumors formed by CMG2 High cells compared to CMG2 Low cells. i The xenograft tumors derived from CMG2 High GC cells showing higher expression of CMG2, CD44, and Ki67 compared to that derived from CMG2 Low cells. Scale bar = 50 μm

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Derivative Assay

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: CMG2 High cells are detected in CD44 High population in GC cell lines and in GC tissues. a Representative flow histogram of the percentage of CD44 High cells in SGC7901 and XN0422 cell lines. b Limiting dilution showing increased capacity of sphere formation by CD44 High cells compared to CD44 Low cells; * P < 0.05. c Representative immunofluorescence images (left panel) and quantitative statistic results (right panel) showing more CMG2-positive cells detected in CD44 High subpopulation than CD44 Low subpopulation; Scale bar = 50 μm; ** P < 0.01. d The co-localization of CMG2 and CD44 in the frozen sections of fresh GC tissues; Scale bar = 100 μm

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Immunofluorescence

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: Silencing CMG2 reduces GCSLC population. a Decreased expression of stemness marker CD44 and stemness-related factor SOX2 in GC cells with CMG2 knockdown (shCMG2). b Representative flow cytometric histograms showing decreased percentage of CD44 High cells in shCMG2 GC cells. c Markedly reduced sphere formation by CMG2 knockdown GC cells; * P < 0.05. d Decreased colony formation by shCMG2 cells. e Representative images showing decreased tumor formation in nude mice by CMG2 knockdown GC cells. f Reduced weight of xenograft tumor formed by shCMG2 GC cells; ** P < 0.01

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Expressing, Marker

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: Silencing CMG2 results in reduced invasive and metastatic properties of GC cells epithelial–mesenchymal transition (EMT). a Representative images of transwell invasion test showing decreased invasion capability of GC cells with knockdown of CMG2. b Quantification of the transwell invasion assay results; Scale bar = 100 μm; ** P < 0.01. c Representative images of intraperitoneal metastasis tests showing reduced number of metastatic foci formed by shCMG2 cells. d Quantification of the intraperitoneal metastasis results; ** P < 0.01. e Western blot showing upregulation of E-cadherin and downregulation of CD44 and vimentin in GC cells with knockdown of CMG2

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Transwell Invasion Assay, Western Blot

Journal: Oncogene

Article Title: Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway

doi: 10.1038/s41388-018-0226-z

Figure Lengend Snippet: CMG2 interacts with LRP6 to activate β-catenin pathway in GC cells. a Representative immunofluorescence images showing co-localization of CMG2 and LRP6 in GC cells; Scale bar = 50 μm. b Co-IP confirmation of physical interaction between CMG2 and LRP6. c Western blotting showing reduced level of nuclear β-catenin in shCMG2 GC cells. d TOP/FOP flash showing decreased transcriptional activity of TCF4 in GC cells with silenced CMG2 (left panel) but increased transcriptional activity of TCF4 in GC cells with overexpressed CMG2 (left panel); * P < 0.05. e Western blot showing attenuated nuclear β-catenin level siLRP6 GC cells. f TOP/FOP flash showing decreased transcriptional activity of TCF4 in siLRP6 GC cells; ** P < 0.01. g Treatment with XAV-939, a specific inhibitor of β-catenin, abolishing CMG2 overexpression-induced TCF4 transcriptional activity; ** P < 0.01. h Treatment with XAV-939 decreasing nuclear accumulation of β-catenin, downregulating CD44 and vimentin, and upregulating E-cadherin in overCMG2 GC cells

Article Snippet: A rabbit monoclonal antibody against LRP6 (Cat. no. ab134146, Abcam) or a goat polyclonal antibody that recognizes all isoforms of CMG2 (Cat. no. SAB2501374, Sigma-Aldrich) or rabbit monoclonal antibody against CD44 (Cat. no. BBA10, R&D) or CK18 (Cat. no. ab82254, Abcam) or H-KATPase (Cat. no. ab2866, Abcam) was added onto the slides.

Techniques: Immunofluorescence, Co-Immunoprecipitation Assay, Western Blot, Activity Assay, Over Expression

Journal: Oncotarget

Article Title: Aquaporin 3 promotes the stem-like properties of gastric cancer cells via Wnt/GSK-3β/β-catenin pathway

doi: 10.18632/oncotarget.7664

Figure Lengend Snippet: Correlations of AQP3 expression or CD44 expression in GC tissues and corresponding non-cancerous mucosa tissues

Article Snippet: Polyclonal rabbit anti-AQP3 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and the monoclonal antibody against CD44 was purchased from Cell Signaling Technology (Beverly, MA).

Techniques: Expressing

Journal: Oncotarget

Article Title: Aquaporin 3 promotes the stem-like properties of gastric cancer cells via Wnt/GSK-3β/β-catenin pathway

doi: 10.18632/oncotarget.7664

Figure Lengend Snippet: Correlation between AQP3, CD44 expression and clinicopathological features in GC

Article Snippet: Polyclonal rabbit anti-AQP3 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and the monoclonal antibody against CD44 was purchased from Cell Signaling Technology (Beverly, MA).

Techniques: Expressing

Journal: Oncotarget

Article Title: Aquaporin 3 promotes the stem-like properties of gastric cancer cells via Wnt/GSK-3β/β-catenin pathway

doi: 10.18632/oncotarget.7664

Figure Lengend Snippet: Correlation between expression levels of AQP3 and CD44 in GC tissues by IHC

Article Snippet: Polyclonal rabbit anti-AQP3 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and the monoclonal antibody against CD44 was purchased from Cell Signaling Technology (Beverly, MA).

Techniques: Expressing

Journal: Oncotarget

Article Title: Aquaporin 3 promotes the stem-like properties of gastric cancer cells via Wnt/GSK-3β/β-catenin pathway

doi: 10.18632/oncotarget.7664

Figure Lengend Snippet: ( A ) Expression levels of AQP3 and CD44 in SGC7901, MGC803, and AGS cells were determined by Western blot method. GAPDH was used as internal control. The relative expression levels of proteins in different groups were compared with those in the null control (NC). ( B ) The mRNA expression levels of AQP3 and CD44 were quantified by RT-qPCR analysis. Data are expressed as the mean ± SE of results from three independent experiments. * P < 0.05 compared with the null control (NC). ( C ) Immunofluorescence assays for the detection of AQP3 and C44. The target proteins were detected using the respective antibodies (red), and nuclei were stained with DAPI (blue). Original magnification, × 200.

Article Snippet: Polyclonal rabbit anti-AQP3 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and the monoclonal antibody against CD44 was purchased from Cell Signaling Technology (Beverly, MA).

Techniques: Expressing, Western Blot, Control, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Oncotarget

Article Title: Aquaporin 3 promotes the stem-like properties of gastric cancer cells via Wnt/GSK-3β/β-catenin pathway

doi: 10.18632/oncotarget.7664

Figure Lengend Snippet: Poor AQP3 and CD44 immunoreactivity in tumors of mice were identified in MGC803-AQP3 low group compared with MGC803-NC group. Strong AQP3 and CD44 immunoreactivity in tumors of mice were identified in AGS-AQP3 high group compared with AGS-NC group. Original magnification, × 400.

Article Snippet: Polyclonal rabbit anti-AQP3 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and the monoclonal antibody against CD44 was purchased from Cell Signaling Technology (Beverly, MA).

Techniques: